A sensitive chemiluminescence assay for pertussis toxin and for evaluation of cell-free pertussis toxoids
نویسندگان
چکیده
منابع مشابه
Development of a qualitative assay for screening of Bordetella pertussis isolates for pertussis toxin production
Bordetella pertussis infection has been increasing in the US, with reported cases reaching over 50,000 in 2012, a number last observed in the 1950s. Concurrently, B. pertussis lacking the pertactin protein, one of the immunogens included in the acellular vaccine formulations, has rapidly emerged since 2010, and has become the predominant circulating phenotype. Monitoring the production of the r...
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Whole-cell pertussis vaccine (wP) has been imperative and highly effective in preventing childhood deaths due to pertussis. Pertussis toxin is one of the virulence factors of Bordetella pertussis in all available pertussis vaccines. wP production in Razi Vaccine and Serum Research Institute is according to bioreactor culture of B. pertussis strains in B2 medium. The aim ...
متن کاملRegulation of pertussis toxin and lipopolysaccharide levels of Bordetella pertussis 134 in response to modulators
Whooping cough (pertussis) is a highly contagious disease of the human respiratory tract, which is caused by Bordetella pertussis. Reemerge of pertussis in some highly immunized populations and divergency in gene order among several B. pertussis strains promoted this research to study the change of pertussis toxin (PT) and lipopolysacharide levels in response to the different environments. Th...
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Serum opsonic capacity against B. pertussis was studied by using quantitative chemiluminescence (CL), a method known to have several advantages over conventional methods in evaluating opsonization and phagocytosis. Sera from unvaccinated infants was shown not to contain opsonins against B. pertussis and in unvaccinated infants suffering from whooping cough, no opsonins were detected. In ad...
متن کاملHighly sensitive histamine-sensitization test for residual activity of pertussis toxin in acellular pertussis vaccine.
Histamine-sensitization test method based on histamine-sensitizing death is widely used for controlling residual activity of pertussis toxin in acellular pertussis vaccines. The test method evaluates the residual activity according to the death of mice injected with a test vaccine after histamine challenge and the test result, therefore, depends on the sensitivity of mice. A highly sensitive te...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 1988
ISSN: 0378-1097
DOI: 10.1016/0378-1097(88)90056-0